214 Topical DEHP exposure leads to inflammation and epidermal barrier damage in epidermal equivalents

Phthalates are quasi-ubiquitous plasticizers found in products including building materials, food packaging, clothes, and personal care products. Thus, we permanently exposed to them. endocrine disruptors that can be ingested or absorbed through the skin. Because DEHP is most common phthalate, sought investigate its effect on epidermis. We generated human epidermal equivalents (HEEs) with keratinocytes from healthy donors treated them topically DEHP. Corneodesmosin was decreased uppermost layers of epidermis, indicating a defect barrier formation. Furthermore, biotin-diffusion assay showed defective tight junctions. Moreover, expression IL1A, IL1B, IL6, IL8 IL18 up-regulated, pro-inflammatory Interestingly, GLUT1 increased as well glucose consumption, suggesting capacity modulate metabolic pathways. LC-MS analysis hair plasticizers, especially phthalates, AD patients. were detectable about 50% patients controls; however, semi-quantitative indicated higher levels particularly DEHP, skin samples further 3.7% all industrial compounds phthalates control group, whereas it reached 15.4% these data show more topical they accumulate, probably due damaged barrier. In vitro experiment leads inflammation damage, hence chronic exposure might contribute development flare-ups.